Recombinant Human IL12B, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P29460 |
| Gene Symbols | IL12B |
| Protein Name / Synonyms | IL12B, Interleukin 12B,NKSF2,Interleukin 12B (Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, P40),Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit ,NK Cell Stimulatory Factor Chain 2,Interleukin-12 Beta Chain,IL-12 Subunit P40 ,CLMF P40 ,IL-12B,Natural Killer Cell Stimulatory Factor, 40 KD Subunit,Natural Killer Cell Stimulatory Factor-2,Cytotoxic Lymphocyte Maturation Factor 2,Natural Killer Cell Stimulatory Factor,Interleukin-12 Subunit Beta,Interleukin 12, P40 ,IL12, Subunit P40,Interleukin 12,40 KD Subunit,Subunit P40,CLMF2,IMD28,IMD29,IL12,CLMF,NKSF,P40 |
| Expressed Region | Ile23-Ser328 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant Human IL12B has a calculated molecular mass of 37 kDa. Due to the abundant glycosylation, it migrates as approximately 35-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Angiogenesis, Cancer, Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
