Recombinant human Insulin protein derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01308 |
| Gene Id | 3630 |
| Gene Symbols | INS |
| Protein Name / Synonyms | Insulin, MODY10, IDDM2, Insulin-Dependent Diabetes Mellitus 2, Preproinsulin, Proinsulin, IDDM1, IDDM, ILPR, IRDN |
| Expressed Region | Phe26-Asn110 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 12 kDa |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bio-Rad protein assay dye reagent concentrate. |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Activity
Stimulating cell growth significantly in a dose-dependent manner. Measured in a cell proliferation assay using MCF‑7 human breast cancer cells.
Measured by its binding ability in a functional ELISA. Immobilized recombinant Insulin Receptor can bind Insulin with EC50 at 355.6 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized recombinant Insulin Receptor can bind Insulin with EC50 at 355.6 ng/mL.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.