P11215-2

Integrin alpha-M, Integrin Alpha M (Complement Component 3 Receptor 3 Subunit), Cell Surface Glycoprotein MAC-1 Subunit Alpha, CD11 Antigen-Like Family Member B, Leukocyte Adhesion Receptor MO1, CR-3 Alpha Chain, CD11B, CR3A, Complement Component Receptor 3 Alpha, Neutrophil Adherence Receptor Alpha-M Subunit, Macrophage Antigen Alpha Polypeptide, Neutrophil Adherence Receptor, Antigen CD11b (P170), CD11b Antigen, MAC-1, MAC1A, SLEB6, MO1A.

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 123 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Culture supernatant of transfected HEK293 cells

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