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Recombinant Human INTERLEUKIN-1RA
Catalog #: 229-20161
Product Description
Specifications
| Size | 20 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Protein Name / Synonyms | IL-1RA, |
| Expression System | E. coli |
| Purity | >98% by SDS PAGE |
| Molecular Weight (kDa) | 17kD/153 amino acids |
| Format | Purified recombinant protein - lyophilized |
| Concentration (lot specific) | Total protein concentration ~0.1mg/ml after reconstitution. |
| Preservative | None present |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays (20-40ng/ml), Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays (20-40ng/ml), Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <0.1ng/ug |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Introduction
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.
In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.
In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.
Specifications
Size
20 ug
Assay Buffers
TRIS buffered saline
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution