O43240

Kallikrein-10, Kallikrein Related Peptidase 10, Normal Epithelial Cell-Specific 1, Protease Serine-Like 1, PRSSL1, NES1, Breast Normal Epithelial Cell Associated Serine Protease, KLK-10

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

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