Recombinant Human Kallikrein-10 (KLK-10), Transfected HEK293 Cell Lysate

Recombinant human kallikrein-10 (KLK-10) protein overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-10006), the cell lysate of HEK293 cells transfected with empty expression vector.

$140.39
230-10296-100
+

Source

Gene Symbols:
  • KLK10
Species:
Human
Accession Number:
  • O43240
Expressed Region:
Ala31-Asn276
Protein Name & Synonyms:
Kallikrein-10, Kallikrein Related Peptidase 10, Normal Epithelial Cell-Specific 1, Protease Serine-Like 1, PRSSL1, NES1, Breast Normal Epithelial Cell Associated Serine Protease, KLK-10

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
C-terminal his-tag
Purity:
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 27 kDa. Due to the abundant glycosylation, it migrates as approximately 35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4) containing mammalian cell protease inhibitor cocktail
Concentration:
Determined by BCA protein assay
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.

Stability & Storage

Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.