Recombinant Human Mitochondrial Serine Protease HTRA2, HEK293 with N-terminal His-tag, derived from HEK293 cells.
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O43464 |
| Gene Id | 27429 |
| Gene Symbols | HTRA2 OMI PRSS25 |
| Protein Name / Synonyms | Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI) |
| Expressed Region | Ala134-Glu458 |
| Expression System | HEK293 cells |
| Tag | N-terminal His-tag |
| Purity | >90 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining. See gel image below. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 37 kDa. Due to the abundant glycosylation, it migrates as approximately 40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial labels. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Protein Concentration Determined By
Determined by Bio-Rad Bradford protein dye reagent concentrate
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.