His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant protein has a calculated molecular weight of about 82 kDa. Due to the abundant glycosylation, it migrates as approximately 90-100 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized powder, slight green
Lyophilized from a 0.2 µm filtered solution in PBS
Determined by Pierce BCA protein assay kit
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.