Recombinant Human Nestin (HEK293 Lysate)

Recombinant human Nestin overexpressed in cell lysate, derived from transfected HEK293 cells (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.

$146.01
230-10022-100
+

Source

Gene Symbols:
  • NES
Species:
Human
Accession Number:
  • P48681
Gene ID:
  • 10763
Expressed Region:
Gln1022-Asp1621, C-terminal
Protein Name & Synonyms:
Nestin

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
Unpurified overexpression cell lysate
Molecular Weight (kDa):
65

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µmicro,m filtered solution in PBS containing mammalian cell protease inhibitor cocktail.
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Specifications

Size
100 ug

Stability & Storage

Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.