P52952-1

NK2 Homeobox 5, transfected HEK293 cell lysate, NK2 Homeobox 5, Homeobox Protein NK-2 Homolog E, Homeobox Protein CSX, NKX2.5, NKX2E, CSX, NK2 Transcription Factor Related, Locus 5 (Drosophila), NK2 Transcription Factor Related, Locus 5, Tinman Paralog (Drosophila), Cardiac-Specific Homeobox 1, Cardiac-Specific Homeo Box, Cardiac-Specific Homeobox, Tinman Paralog, NKX4-1, CHNG5, HLHS2, CSX1, VSD3

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Lyophilized powder

Determined by Pierce BCA protein assay kit