Recombinant human NMDA 2B protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q13224 |
| Gene Symbols | GRIN2B |
| Protein Name / Synonyms | GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, N-methyl-D-aspartate receptor subunit 3, NR3, Hnr3. |
| Expressed Region | Hybrid peptides (AA: Arg27-Asp557, Tyr579-Val634, Gln656-Asn817) |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 85 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼85 kDa band. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
