Recombinant human NT-proBNP, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P16860 |
| Gene Id | 4879 |
| Gene Symbols | NPPB |
| Protein Name / Synonyms | NT-proBNP, Natriuretic peptides B, Natriuretic Peptide B, Brain Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-Brain Natriuretic Peptide, Natriuretic Protein, BNP |
| Expressed Region | His27-Arg102 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant human NT-proBNP has a calculated molecular mass of 35 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
N-Terminal pro-B-Type Natriuretic Peptide is a biomarker derived from the cleavage of proBNP, which is produced and secreted primarily by cardiomyocytes in response to increased wall stress due to volume overload or elevated intracardiac pressure. It is predominantly expressed in the heart, particularly the ventricles. NT-proBNP is widely used in the clinical assessment of heart failure, as its levels correlate with cardiac dysfunction and can help guide diagnosis and treatment. Unlike B-type natriuretic peptide (BNP), NT-proBNP has a longer half-life, making it a more stable marker for evaluating cardiac function.
Reference: https://www.mdpi.com/2308-3425/11/7/225
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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