NP_000573

Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, OPN, Early T-Lymphocyte Activation 1, Nephropontin, BNSP, Bone Sialoprotein I, SPP1/CALPHA1 Fusion, Bone Sialoprotein 1, ETA-1, BSPI, Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1) Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion Protein

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Pink liquid

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