5295

Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Phosphoinositide-3-Kinase Regulatory Subunit 1 (Alpha), Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha, PI3-Kinase Subunit P85-Alpha, GRB1, Phosphatidylinositol 3-Kinase-Associated P-85 Alpha, Phosphoinositide-3-Kinase Regulatory Subunit, PtdIns-3-Kinase Regulatory Subunit P85-Alpha, P85-ALPHA, IMD36, AGM7, P85.

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 50 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Lyophilized from a 0.2 µm filtered solution,in PBS containing mammalian cell protease inhibitor cocktail

None