Product Description

Specifications

Size	10 µg, 50 µg, 100 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	P01106
Gene Id	4609
Gene Symbols	MYC
Protein Name / Synonyms	Myc proto-oncogene protein, Transcription factor p64
Expressed Region	Pro2-Ala439
Expression System	E. coli
Tag	N-terminal 6x histidine tag
Purity	> 85%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	~65 kDa. The predicted size is ~50
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS.
Preservative	None
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level	Not determined
Research Area	Cancer
Shipping Type	Ambient temperature
Storage	-20°C

Introduction

The MYC proto-oncogene encodes a 439-amino acid nuclear phosphoprotein that functions as a transcription factor, regulating various cellular processes such as cell cycle progression, apoptosis, and metabolism. MYC is primarily expressed in proliferating cells, where it forms heterodimers with its partner protein MAX, allowing it to bind specific DNA sequences known as E-boxes and modulate gene expression. Under normal physiological conditions, MYC expression is tightly regulated; however, its dysregulation and overexpression are implicated in the development of numerous human cancers. Notably, MYC overexpression can enhance interactions with lower-affinity E-boxes, leading to tumorigenesis by altering gene activation patterns that regulate cell proliferation and growth. Recombinant human MYC protein is utilized extensively in research to study its role in oncogenesis and to develop potential therapeutic interventions targeting its activity.

Reference: https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-020-01291-6#:~:text=Abstract

SDS-PAGE Image

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant c-MYC at 1 µg/mL (100 µL/well) can bind Human KRT8 with EC50 at 2.1 µg/mL.

Measured by its binding ability in Auto-western blotting. Recombinant human proto-oncogene c-Myc protein can bind anti-c-Myc tag monoclonal antibody. Left lane, protein ladder (kDa); right lane, reaction band (> 65 kDa).

Measured by its binding ability in a transcription factor ELISA. Immobilized DNA containing the cMyc consensus binding sequence (CACGTG) can bind human cMyc with an EC50 of 4.668 ng/mL.

Storage/Stability

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.