Recombinant human ROBO1 protein, N-terminal region with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q1RMC8 |
| Gene Symbols | ROBO1 |
| Protein Name / Synonyms | Roundabout Guidance Receptor 1, Deleted In U Twenty Twenty, DUTT1, Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila), Roundabout (Axon Guidance Receptor, Drosophila) Homolog 1, Roundabout, Axon Guidance Receptor, Homolog 1, H-Robo-1, SAX3 |
| Expressed Region | Ser20-Pro861 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼95kDa. Due to the abundant glycosylation, it migrates as approximately ∼110 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼95 kDa band. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under denature conditions.
Lane 5: Deglycosylation enzymes only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
