Biotin-conjugated recombinant human S100-A11 protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P31949 |
| Gene Symbols | S100A11 |
| Protein Name / Synonyms | Protein S100-A11, Calgizzarin, MLN 70, S100 calcium-binding protein A11 |
| Expressed Region | Ala2-Thr105 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 14 kDa. Due to the abundant glycosylation, it migrates as approximately 18 kDa extra protein band (above 14 kDa band) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single ~14 kDa band. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
