Recombinant Human SPARC/BM40, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P09486 |
| Gene Symbols | SPARC |
| Protein Name / Synonyms | SPARC, Secreted Protein Acidic And Cysteine Rich, Secreted Protein Acidic And Rich In Cysteine, Basement-Membrane Protein 40, Osteonectin, BM-40, ON, Secreted Protein, Acidic, Cysteine-Rich, Cysteine-Rich Protein, OI17, SPARC, Secreted protein acidic and rich in cysteine |
| Expressed Region | Ala18-Ile303 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >70% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant Human SPARC/BM40 has a calculated molecular mass of 35 kDa. Due to the abundant glycosylation, it migrates as approximately 40-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. Some large proteins were copurified due to the potential protein-protein interaction. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Angiogenesis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Shipping
Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.Ã For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
