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Source
Gene Symbols:
- TNFA
Species:
Human
Accession Number:
- P01375
Gene ID:
- 7124
Expressed Region:
Val77-Leu233
Protein Name & Synonyms:
TNFa, TNF-alpha, Tumor necrosis factor
Preparation
Expression System:
E.Coli
Conjugation/Tag:
C-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
17
Protein Specifications
Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4).
Concentration:
Lot specific (see the label on the vial)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image
Data Images
Figure 1
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243). After stimulation with TNF-α, activated NF-κB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-κB p65 from cytoplasm and nuclear fractions; B. Transcription factor activity assay of NF-κB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).
Figure 2
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243) with the specific competitor or non-specific competitor. The result shows specific binding of NF-κB p65 to the NF-κB DNA binding site.
Figure 3
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells treated with TNF-α (RayBio Cat# 230-00243) and purified NF-κB p50. No cross-reactivity has been observed between NF-κB p65 and p50.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
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Bauer D., Redmon N., Mazzio E., et al. Diallyl disulfide inhibits TNF? induced CCL2 release through MAPK/ERK and NF-Kappa-B signaling. Cytokine. 2015 Sep;75(1):117-26. doi: 10.1016/j.cyto.2014.12.007.
Species: Human
Sample type: N/A -
Bauer D., Mazzio E., Soliman K., Taka E., Oriaku E., et al. Diallyl Disulfide Inhibits TNF?-induced CCL2 Release by MDA-MB-231 Cells. ANTICANCER RESEARCH 34: 2763-2770 (2014)
Species: Human
Sample type: Conditioned Media -
Wang S., Ohrmund L., Hauenstein S., et al. Development and validation of a homogeneous mobility shift assay for the measurement of infliximab and antibodies-to-infliximab levels in patient serum. J Immunol Methods. 2012 Aug 31;382(1-2):177-88. doi: 10.1016/j.jim.2012.06.002.
Species: Human
Sample type: N/A
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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