Recombinant Human TNFRSF11B Protein, Biotinylated

Name: Recombinant Human TNFRSF11B Protein, Biotinylated
SKU: 230-30269B-50
Availability: InStock

Biotin-conjugated recombinant human tumor necrosis factor receptor superfamily member 11B/TNFRSF11B with a C-terminal His-tag, derived from the transfected human HEK293 cells.

Product Description

Specifications

Size	50 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	O00300
Gene Symbols	TNFRSF11B
Protein Name / Synonyms	Osteoclastogenesis inhibitory factor, Osteoprotegerin.
Expressed Region	Glu22-Leu401
Expression System	HEK293 cells
Conjugation	Biotin
Tag	C-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Purity	>90%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	Recombinant protein product has a calculated molecular mass of 45 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose
Concentration (lot specific)	Prior to lyophilization, the protein concentration was measured by Bio-Rad Bradford protein dye reagent concentrate.
Reconstitution	Briefly spin the vial to ensure all contents are at the bottom before opening. It is recommended to reconstitute the product at a concentration of 0.5-1.0 mg/mL using sterile deionized water.
Endotoxin Level	< 0.5 EU per μg of the protein as determined by the LAL method
Research Area	Cancer, Inflammation, Necrosis
Shipping Type	Blue ice
Storage	-20°C

SDS-PAGE Image

Figure 1. Deglycosylation analysis of purified recombinant proteins. Product was either untreated (Lane 2) or treated with deglycosylation enzymes under native (Lane 3) or denaturing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift, indicating that the untreated protein (Lane 2) was glycosylated. Lane 1, protein ladder (kDa).

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse TNFSF11 at 2 µg/mL (100 µL/well) can bind human TNFRSF11B with EC50 at 0.25 µg/mL.

Storage/Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store the desiccated product below -20°C in a manual defrost freezer. Generally, the shelf life is up to 12 months from the date of receipt when stored at -20°C or -80°C under sterile conditions. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to prepare appropriate aliquots and store them at -20°C or -80°C.