Recombinant Human Troponin T type 2 (cardiac), E.coli-derived
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P45379 |
| Gene Id | 7139 |
| Gene Symbols | TNNT2 |
| Protein Name / Synonyms | TnTc, Cardiac muscle troponin T, cTnT. |
| Expressed Region | Ser2-Lys298 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The predicted molecular weight is 36.5 kDa. The actual recombinant protein migrates as approximately 45 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5. Protein concentration prior to lyophilization was 0.25 mg/mL. |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers. |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
