Lyophilized from a 0.2 um filtered solution in 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5. Protein concentration prior to lyophilization was 0.25 mg/mL.
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array
Protein Name & Synonyms:
TnTc, Cardiac muscle troponin T, cTnT.
N-terminal 6x histidine tag
Molecular Weight (kDa):
The predicted molecular weight is 36.5 kDa. The actual recombinant protein migrates as approximately 45 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.