Lyophilized from a 0.2 µm filtered solution in PBS
Preservative
None
Protein Name / Synonyms
TPD52L2, Tumor protein D54, hD54, Tumor protein D52-like 2, Tumor Protein D52 Like 2, HCCR-Binding Protein 2, TPD54, D54
Accession Number
O43399
Gene Symbols
TPD52L2
Recommended Applications
Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Concentration (lot specific)
Determined by Pierce BCA protein assay kit
Purity
>95%
Purity Determined By
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Expressed Region
Asp2-Phe206
Expression System
Human Embryonic Kidney 293 Cells
Molecular Weight
Recombinant Human TPD52L2 has a calculated molecular mass of 24 kDa. Due to the abundant glycosylation, it migrates as approximately 35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa). Lane 2: Untreated protein under reducing conditions. Lane 3: Treated protein with deglycosylation enzymes under native conditions. Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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