Recombinant Monkeypox Virus Envelope protein A28 homolog, A30L

Recombinant monkeypox virus (MPXV) envelope protein A28 homolog with C-terminal His-tag, derived from the transfected human HEK293 cells.

$312.90  
230-30235-100
+

Product Specifications

Size:
100 ug
Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Determined by BCA protein assay
Purity:
>95%
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level:
<0.5 EU per µg of the protein as determined by the LAL method
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Source

Gene Symbols:
  • A30L
Species:
Monkeypox Virus (MPXV)
Accession Number:
  • Q8V4U9
Expressed Region:
Gln22-Leu146

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Tag:
His
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼14 kDa. Due to the abundant glycosylation, it migrates as approximately ∼20-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
SDS-PAGE:
SDS-PAGE
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.