Filtered solution in PBS with 1% mannitol and 5% trehalose
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Bio-Rad protein assay reagent
<0.5 EU per µg of the protein as determined by the LAL method
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Monkeypox Virus (MPXV)
Human embryonic kidney 293 (HEK293) cells
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼13 kDa. Due to the abundant glycosylation, it migrates as two major bands: approximately ∼13 kDa non-glycosylated form and ∼16 kDa glycosylated form in SDS-PAGE under DTT and beta-mercaptoethanol reducing conditions.
Stability & Storage
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.