Recombinant mouse interleukia IL-16, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Accession Number | O54824 |
| Gene Id | 16170 |
| Gene Symbols | Il16 |
| Protein Name / Synonyms | Pro-interleukin-16 [Cleaved into: Interleukin-16 (IL-16) (Lymphocyte chemoattractant factor) (LCF)], Interleukin 16, IL-16 |
| Expressed Region | Ser1205-Ser1322 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the post-translation modifications, it migrates as approximately 14-20 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein sizes slightly decreased. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | Not determined |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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