Biotin-conjugated recombinant mouse Nanog, derived from the transfected human HEK293 cells.
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Recombinant Mouse Nanog Protein, Biotinylated
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Mouse |
| Accession Number | Q80Z64 |
| Gene Symbols | Nanog |
| Protein Name / Synonyms | Mouse Nanog Protein |
| Expressed Region | Ser2-Ile305 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >85% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant mouse Nanog protein has a calculated molecular mass of 35 kDa. Due to the abundant glycosylation, it migrates as approximately 75 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
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Recombinant Mouse Nanog Protein, Biotinylated