Recombinant Sus scrofa Cystatin B protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Sus scrofa |
| Accession Number | Q29290 |
| Gene Symbols | CSTB |
| Protein Name / Synonyms | Cystatin-B, Cystatin B, Liver Thiol Proteinase Inhibitor, Cystatin B (Stefin B), CPI-B, STFB, CST6, Stefin B, Stefin-B, EPM1A, EPM1, PME, ULD. |
| Expressed Region | Met2-Phe98 |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 11 kDa. Due to the abundant glycosylation, it migrates as approximately 18 kDa extra protein band (beside 11 kDa band) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation, the protein presented as one single 11 kDa band. See deglycosylation analysis in SDS-PAGE image. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein under reducing conditions.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Lane 5: deglycosylation mixture only without target proteins.
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
