Recombinant Pyruvate Oxidase (PYO) Protein produced in E.coli is a His-tagged protein having a theorectical molecular mass of 66 kDa, purified by proprietary chromatographic techniques.
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Bacteria |
| Gene Symbols | poxB |
| Protein Name / Synonyms | POX, PoxB, Pyruvate:oxygen oxidoreductase |
| Expressed Region | Full length |
| Expression System | E. coli |
| Tag | C-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | PYO has theorectical molecular weight of 66 kDa. Smaller PYO speices 50-60 kDa are observed perhaps due to alternative translational start site. |
| Format | White powder |
| Formulation | Lyophilized powder from sterile filtered PBS, pH 7.4 |
| Activity | Specific Activity >0.4 U/mg |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.