Biotin-conjugated recombinant Rat Angiotensinogen protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Rat |
| Accession Number | P01015 |
| Gene Id | 24179 |
| Gene Symbols | Agt Serpina8 |
| Protein Name / Synonyms | Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II); Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] |
| Expressed Region | Asp25-Val477 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 50 kDa. Due to the abundant glycosylation, it migrates as approximately 55-60 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Filtered 0.2 µm solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial label, determined by BCA protein assay reagent. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.