Recombinant rat IL-6 protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Rat |
| Accession Number | P20607 |
| Gene Symbols | IL6 |
| Protein Name / Synonyms | Interleukin-6, B-Cell Stimulatory Factor 2, CTL Differentiation Factor, Hybridoma Growth Factor. |
| Expressed Region | Phe25-Thr211 |
| Expression System | HEK293 cells |
| Tag | N-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 20 kDa. Due to the abundant glycosylation, it migrates as approximately 20-22 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.