Recombinant rat TNFa derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Rattus norvegicus |
| Accession Number | P16599 |
| Gene Symbols | TNFA |
| Protein Name / Synonyms | Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form. |
| Expressed Region | Gly57-Leu235 |
| Expression System | E. coli |
| Tag | N-terminal histidine tag |
| Purity | >90 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 20 kDa |
| Format | Liquid |
| Formulation | Filtered solution in 20 mM NaH2PO4 (pH 7.4), 0.5 M NaCl, 800 mM imidazole, 10% glycerol. |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bradford protein assay. |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Activity
Decreasing cell viability significantly. Measured in a cytotoxicity assay using MCF‑7 human breast cancer cells.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.