Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | SARS-CoV-2 |
| Accession Number | QHD43423 |
| Protein Name / Synonyms | Nucleocapsid Protein, N Protein |
| Expressed Region | Met1-Ala419 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >90 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SARS-CoV-2 relies on its nucleocapsid (N) protein to package and protect its RNA genome within the viral particle. Beyond its role in viral replication and assembly, the N protein also interacts with host immune signaling pathways, potentially modulating immune responses and disease severity. Due to its high immunogenicity, it is widely used as a diagnostic target for detecting SARS-CoV-2 infection.
Reference: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012886
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions. Shown one ˜55 kDa major band (red arrow) and 25-30 kDa multiple of minor bands (green arrows). This small bands are likely cleavage products. All bands were confirmed by Western blotting (Lane 5). Other minor large bands (>80 kDa) may be the trace amount of copurified proteins from host cells.
Lane 3: Treated protein with deglycosylation enzymes under native conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 5: Western blotting analysis of Lane 1. Shown two major reaction bands (red arrow, green arrow).
Activity
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
