Recombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

$335.25  
230-30164-100
+

Product Specifications

Size:
100 ug, 500 ug, or 1000 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>90%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.
Sandwich ELISA: this protein functions as a standard in conjunction with 130-10781 (capture antibody) and 130-10761 (detection antibody).
Standard curve

Source

Species:
SARS-CoV-2
Accession Number:
  • QHD43423
Expressed Region:
Met1-Ala419
Protein Name & Synonyms:
Nucleocapsid Protein, N Protein

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below.
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (47 kDa), thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions. Shown one ∼55 kDa major band (red arrow) and 25-30 kDa multiple of minor bands (green arrows). This small bands are likely cleavage products. All bands were confirmed by Western blotting (Lane 5). Other minor large bands (>80 kDa) may be the trace amount of copurified proteins from host cells.
Lane 3: Treated protein with deglycosylation enzymes under native conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 5: Western blotting analysis of Lane 1. Shown two major reaction bands (red arrow, green arrow).

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit
Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S1 Subunit Protein with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag
It works well for fluorescent labelling and confocal microscopy.

University of California, Davis
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.