Biotin-conjugated recombinant Streptomyces avidinii streptavidin protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Bacteria, Streptomyces avidinii |
| Accession Number | P22629 |
| Protein Name / Synonyms | Streptavidin |
| Expressed Region | Asp25-Gln183 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | Native PAGE under non-reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 16.5 kDa in monomer. Under native PAGE, it formed polymer and migrated as approximately 120 kDa polymer protein band. |
| Format | Liquid |
| Formulation | Filtered 0.2 um solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial label, determined by BCA protein assay reagent. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.