Biotin-conjugated recombinant Sus scrofa (pig) interleukin-4 (IL-4) protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Sus scrofa |
| Accession Number | Q04745 |
| Gene Symbols | IL4 |
| Protein Name / Synonyms | Interleukin-4, Interleukin 4, IL-4, Lymphocyte Stimulatory Factor 1, B_cell Stimulatory Factor 1, B Cell Growth Factor 1, Binetrakin, Pitrakinra, BSF-1, B-Cell Stimulatory Factor 1, Interleukin 4 Variant 2, BCGF-1, BCGF1, BSF1. |
| Expressed Region | His25-Cys133 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 12 kDa. Due to the abundant glycosylation, it migrates as approximately 12-25 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Inflammation, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
