Recombinant Porcine (pig) Pepsin A Protein, HEK293 lysate
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Sus scrofa |
| Accession Number | P00791 |
| Gene Symbols | PGA |
| Protein Name / Synonyms | Pepsin A |
| Expressed Region | Ile60-Ala385 |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 40 kDa. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs). |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
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Recombinant Porcine (pig) Pepsin A Protein, HEK293 lysate