Recombinant Sus scrofa (pig) Troponin C, Transfected HEK293 Cell Culture Supernatant

Recombinant Sus scrofa (pig) troponin C protein overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-20001), the culture supernatant of HEK293 cell transfected with empty expression vector.

$120.19
230-20324-200
+

Source

Gene Symbols:
  • TNNC1
Species:
Sus scrofa (pig)
Accession Number:
  • P63317
Expressed Region:
Asp2-Glu161
Protein Name & Synonyms:
Troponin C, slow skeletal and cardiac muscles, Troponin C1, Slow Skeletal And Cardiac Type, Troponin C Type 1 (Slow), TN-C, TNNC, Slow Twitch Skeletal/Cardiac Muscle Troponin C, Cardiac Troponin C, CMD1Z, CMH13, TNC.

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
N-terminal his-tag
Purity:
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 18. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs).

Protein Specifications

Format:
Pink liquid
Formulation:
Cell culture supernatant of transfected HEK293 cells, serum-free.

Stability & Storage

Shipping
Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.