Recombinant Sus scrofa (pig) Troponin C, Transfected HEK293 Cell Lysate

Recombinant Sus scrofa (pig) troponin C protein overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-10006), the cell lysate of HEK293 cells transfected with empty expression vector.


Product Specifications

100 ug


Gene Symbols:
  • TNNC1
Sus scrofa (pig)
Accession Number:
  • P63317
Expressed Region:
Protein Name & Synonyms:
Troponin C, slow skeletal and cardiac muscles, Troponin C1, Slow Skeletal And Cardiac Type, Troponin C Type 1 (Slow), TN-C, TNNC, Slow Twitch Skeletal/Cardiac Muscle Troponin C, Cardiac Troponin C, CMD1Z, CMH13, TNC.


Expression System:
Human embryonic kidney 293 (HEK293) cells
N-terminal his-tag
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 18. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs).

Protein Specifications

Lyophilized powder
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4) containing mammalian cell protease inhibitor cocktail
Determined by BCA protein assay
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.

Stability & Storage

Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.