Use code SIMOA26 to receive 15% off all Simoa services through February 2027.
Simoa® - Single Molecule Array Services
When ultrasensitivity is critical to your experiment’s success, RayBiotech’s experienced service team can use the Simoa® (single molecule array) protein detection platform to help you find that needle in a haystack.
With our Simoa assay service, we can achieve orders of magnitude more sensitivity than standard sandwich-based immunoassay techniques, detecting as little as femtogram (fg/mL) concentrations, while consuming as little as a few microliters of sample. This ultrasensitive detection capability enables the detection and quantification of biomarkers at concentrations that have previously been difficult or impossible to measure.
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Ready to send your samples? Please complete the SIMOA Sample Service Form and review the Sample Shipment Instructions.
Already have a Simoa instrument? RayBiotech provides affordable, Simoa-validated antibody pairs for your in-lab use. Browse our antibody pairs.
Why You Should Choose RayBiotech for Simoa Services
For Our Multiplexing Capability
If you need multi-analyte detection, RayBiotech can multiplex up to 4 analytes per test, without requiring any additional sample. In addition to our popular neurological panel (Aβ40, Aβ42, GFAP, NfL), we also provide panel customization to your specifications.
For Our Extensive Catalog of Validated Antibody Pairs
If you don’t see your target in the list of assays below, we can convert any of our validated ELISA antibody pairs into the ultrasensitive Simoa assay format. Browse our ELISA target list. If you prefer to run your own assay, browse our antibody pairs, including a complete list of ready-to-use Simoa antibody pair kits.
For Our Years of Experience
Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding protein detection and analysis services to the research community.
Data From Our Simoa Lab, Published
See how researchers used our Simoa service, from Alzheimer’s patient plasma to mouse tumor tissue.
FEATURED STUDY · ALZHEIMER’S DISEASE
Tracking NfL and GFAP in Alzheimer’s patients treated with lecanemab
Two 2026 real-world studies followed Japanese Alzheimer’s disease patients receiving lecanemab, examining ATNIVS biomarker heterogeneity and amyloid-related imaging abnormalities (ARIA). RayBiotech measured plasma NfL and GFAP from the study biobank on the Simoa HD-X using the Neurology 2-Plex B assay.
“...Simoa HD-X platform and the Neurology 2-Plex B multiplex assay ... at Raybiotech, Inc.”
From the methods section, J Prev Alzheimers Dis, 2026
Sample | Human plasma |
Assay | Neurology 2-Plex B (N2PB) |
Analytes | NfL, GFAP |
Cohort | Real-world lecanemab patients |
Two papers from this cohort:
ATNIVS biomarker heterogeneity in real-world patients receiving lecanemab
How Simoa Works
Simoa is an ultrasensitive ELISA that uses antibody-coated beads, a fluorescently conjugated detection antibody, and digital readout. After the beads, sample, and detection antibody have been mixed together, the solution is applied to a cartridge with over 235,000 microwells. Each microwell can hold only one bead. Oil is then added to the cartridge, which pushes the sample across the surface of the microwell array and forms a liquid-tight seal of the wells.
The fluorescence of each well is measured using a fully or semi-automated system equipped with a fluorescent microscope. The instrument is manufactured by the developer of Simoa(Quanterix; Billerica, MA; USA). Quantitation of target protein concentration is based on the number of microwells with fluorescent signal and not the intensity of the fluorescent signal, making the readout a "digital" one. Since each well can contain only one bead, the number of microwells with fluorescence is proportional to the amount of target protein in the sample. This approach enables quantitative, ultrasensitive single molecule detection.
Available Assays
Check out our list of available analytes. Multiplexing is also available for up to 4 analytes.
Custom Targets
Don’t see the target you’re looking for? We have a library of thousands of ELISA antibodies which can be converted to the ultrasensitive Simoa assay format. Browse our target list here. Then fill out our Request a Quote form with the target you’re interested in and we will contact you with a quote. Please note: the conversion requires an additional 4-6 week lead time.
See What Simoa Sensitivity Can Do For Your Research
To get started, fill out our Request a Quote form with the targets you’re interested in. We’ll get back to you as soon as possible.
Still have questions?
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