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ISO 13485:2016 cGMP
Staphylococcal Protein-A Cys Recombinant
Catalog #: 228-22030
Product Description
Specifications
| Size | 100 mg, 1 g |
| Estimated Lead Time | 2-3 weeks |
| Species | Bacteria |
| Accession Number | P38507 |
| Protein Name / Synonyms | Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA. |
| Expressed Region | NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by: , (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | SPA protein was lyophilized with no additives. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
SPA-Cys Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 297 amino acids and having a molecular mass of 33.5kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
Stability & Storage
Storage/Stability
Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.