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ISO 13485:2016 cGMP
Staphylococcal Protein-A Recombinant, His Tag
Catalog #: 228-22033
Product Description
Specifications
| Size | 100 mg, 1 g |
| Estimated Lead Time | 2-3 weeks |
| Species | Bacteria |
| Accession Number | P38507 |
| Protein Name / Synonyms | Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA. |
| Expressed Region | MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH. |
| Expression System | E. coli |
| Purity | Greater than 97.0% as determined by: (a) Analysis by HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | SPA protein was lyophilized with no additives. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
SPA Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain fused with 6ÃHis tag at C-terminus. SPA is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 306 amino acids and having a molecular mass of 34.7kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
Stability & Storage
Storage/Stability
Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.