This product is a 2X premixed reagent specifically designed for real-time PCR using the SYBR Green I intercalating dye method. This product contains a universal ROX reference dye, making it compatible with all qPCR instruments.
This product is a 2X premixed reagent specifically designed for real-time PCR using the SYBR Green I intercalating dye method. The mix features double-sealed HotStart Taq DNA Polymerase, combined with a specific dual-cation buffer and various enhancers to improve specificity and reduce primer dimer formation, resulting in more accurate and reliable results. This product contains a universal ROX reference dye, making it compatible with all qPCR instruments. No ROX concentration adjustment is necessary for different instruments; simply add primers and template to the reaction mixture for amplification.
Typical Data
Figure 1. Performance evaluation of the SYBR Green qPCR Mix shows high sensitivity, specificity, and reproducibility across a wide dynamic range. qPCR amplification of the human IFNB1 gene was performed on a QuantStudio™ 5 Real-Time PCR System using genomic DNA isolated with the Universal Genomic DNA Extraction Kit (Catalog #540-20001). Serial dilutions of 293T genomic DNA (0.005–200 ng) were tested in duplicate. (A) Amplification curves demonstrate consistent, concentration-dependent amplification with low replicate variability. (B) Melt curve analysis shows a single, sharp peak, indicating specific target amplification without detectable primer-dimer formation. (C) Agarose gel electrophoresis confirms the expected amplicon size across input DNA concentrations. Figure 2. Comparative amplification efficiency of the SYBR Green qPCR Mix. The SYBR Green qPCR Mix (RayBio) and two commercially available master mixes were evaluated using serial dilutions of 293T genomic DNA (5 pg/mL–200,000 pg/mL). (A) Cq values across template concentrations with corresponding log₁₀-transformed inputs and calculated PCR efficiencies. (B) Standard curves generated from Cq values versus log₁₀ DNA concentration, showing strong linearity and efficient amplification performance across the dynamic range.
Storage/Stability
Store at -20°C, protected from light. Before use, thaw completely and mix thoroughly. Avoid repeated freeze-thaw cycles. Stable for 12 months if stored properly.
Performance evaluation of the SYBR Green qPCR Mix shows high sensitivity, specificity, and reproducibility across a wide dynamic range.
SYBR Green qPCR Mix
Rating
No issues with the product
Review by Paula Oliva Ribas, from Harvard - The Joshi Lab, on May 8, 2026
$80.00
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SYBR Green qPCR Mix (2X, Universal ROX)
This product is a 2X premixed reagent specifically designed for real-time PCR using the SYBR Green I intercalating dye method. This product contains a universal ROX reference dye, making it compatible with all qPCR instruments.
