The RayBiotech Total Cholesterol Colorimetric Assay Kit is a straightforward, reliable, and highly sensitive tool for measuring the concentration of total cholesterol, which encompasses both cholesteryl esters and free cholesterol, in plasma, serum, cell lysates and other biological liquid samples.
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Energy Metabolism, Obesity & Metabolic Disease, Glycolysis & Lipid Metabolism |
| Shipping Type | Blue ice |
| Storage | 2-8°C |
Cholesterol plays a crucial role in various physiological processes, including the composition of cell membranes, hormone production, and cell signaling. As a vital component of animal cell structure, cholesterol is essential for maintaining membrane permeability and fluidity, as well as contributing significantly to cell signaling pathways. Monitoring cholesterol levels is critical for diagnosing and classifying hyperlipoproteinaemias, as it can provide valuable insights into liver and biliary function, intestinal absorption, susceptibility to coronary artery disease, thyroid and adrenal function.
The RayBiotech Total Cholesterol Colorimetric Assay Kit offers a straightforward, reliable, and highly sensitive method for measuring the concentration of total cholesterol, which encompasses both cholesteryl esters and free cholesterol, in plasma, serum, cell lysates and other biological liquid samples. This assay relies on an enzymatic reaction system that couples several essential steps. First, cholesterol esters are enzymatically hydrolyzed by cholesterol esterase, resulting in the conversion of cholesteryl esters into cholesterol and free fatty acids. Subsequently, the cholesterol, including the original content, is oxidized by cholesterol oxidase to produce cholest-4-en-3-one and hydrogen peroxide (H2O2). Finally, a Colorimetric Probe reacts with the generated hydrogen peroxide, forming a pink-colored product. The optical density measured at 500nm is directly proportional to the concentration of cholesterol within the sample.
| Component | Size / Description |
|---|---|
| Microplates | A 96-well (12 strips x 8 wells) plate |
| Sample Buffer | 10 ml |
| Cholesterol Standard | 1 vial (100 µl of 5172 µM) |
| Enzyme Mix Solution | 20 ml |
Each cholesterol standard and sample should be assayed in duplicate or triplicate. A standard curve should be generated each time the assay is performed.
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The RayBiotech Total Cholesterol Colorimetric Assay Kit is a straightforward, reliable, and highly sensitive tool for measuring the concentration of total cholesterol, which encompasses both cholesteryl esters and free cholesterol, in plasma, serum, cell lysates and other biological liquid samples.
Total Cholesterol Assay Kit (Colorimetric)
Total Cholesterol Assay Kit (Colorimetric)
The kit is easy to use. We used it on animal serum, so we get the data quickly.
Review by Carol, on Jul 29, 2026
I used this sample to measure total cholesterol in serum samples from mice fed with either a normal chow or high fat diet. The total cholestrol levels almost doubled in the high fat diet group. I used only 1 microliter of samples. The kit is easy to use and sensitive.
Review by Dan, from Baylor College of Medicine, on Aug 13, 2025
This is a very good kit. I have been using this for past few months. The protocol is very easy and takes very less time. Highly recommended. I am using this kit to check the cholesterol content and quantitatively analyse the foam cell formation. I am getting reproducible results with every use.
Review by Rituparna Chakrabarti, from Johns Hopkins school of medicine, on Apr 21, 2025
We used conditioned medium to test this assay, the protocol was simple and straightforward.
Review by Core User, from University of Kansas Medical Center, on Mar 26, 2025
My lab used this assay on rabbit plasma and had no problems! It gave us data we were expecting. The assay is super simple and easy to use which is great. Everything you need is in the kit and instructions are very clear and easy to follow. We did run samples at half sample size (5ul sample instead of 10ul) and half of Enzyme mix solution to test for reactivity before running a full assay (100ul instead of 200ul) and it still worked great. The standard was also very easy to prepare, simply serial dilution, also worked really well. Although, it would have been optimal if there was more standard given as we ran different samples on different plates and would have liked to run the standards side by side on each run just to be sure. Other than that, very good assay! would recommend and definitely use again
Review by Jackie, from University of Illinois at Chicago, on May 21, 2024
I've used this total cholesterol quantification kit on mammalian plasma (non-human, non-rodent). It is quick and easy to use, simply pipette the assay solution in the plate wells, add samples or standards, incubate a few minutes and read. I didn't have to dilute my samples to fall in range of the standard curve, which is a plus for me. There is even a strip plate and a few plate seals included in the kit. I just wish there was more standard provided to make it easier to run more partial plates. Still, I will buy again when I need more as the price is very competitive compared to similar kits from other vendors. Another great product from RayBiotech.
Review by Mario, from Stealth Startup, on Jan 31, 2024
Samples used: Purified Cholesterol Solution from Sigma Conditions: Increasing concentrations of cholesterol with and without cholesterol chelator (cyclodextrin) The kit was very easy to use! However, for cell culture work and the microgram per mL concentrations we will be testing, this kit cannot detect that low. We adjusted our standard curve to match our conditions without cells, and kit could not detect. It is okay! This kit is just not for us.
Review by AstroLab, from CSMC, on Dec 27, 2023
