3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Trypsin, Purified, Sequencing Grade II
Catalog #: 252-10154
Bovine trypsin that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity and extensively purified to remove autolysis products, resulting in a highly stable trypsin product resi…
Product Description
Specifications
| Size | 4x25 µg, 4x100 µg, 1 mg |
| Estimated Lead Time | 1-2 weeks |
| Accession Number | P00760 |
| Expression System | Bovine Pancreas |
| Molecular Weight (kDa) | 23.3 kDa (Theoretical) |
| Format | Lyophilized (powder) |
| Activity | ≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein) |
| Recommended Applications | Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglu, Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglutination procedures Sample preparation for flow cytometric DNA analysis Tryptic mapping Fingerprinting and sequencing work Environmental monitoring Reduction of cell density in tissue culture Subculturing cells Cleavage fusion proteins Generating glycopeptides from purified glycoproteins (White and White 1997) |
| Research Area | Energy Metabolism |
| Source | Bovine Pancreas |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Other Information
Protease sequencing grade
Yes
Extinction Coefficient
Trypsinogen: 45,250 cm-1 M-1>
Isoelectric
Trypsinogen: pH 9.3 (Walsh and Neurath 1964) Trypsin: pH 10.5 (Cunningham 1954).
CATH
Class: Mainly Beta Architecture: Beta Barrel Topology: Thrombin, subunit H
CAS Registry Number
C.A.S.: 9002-07-7
Storage Conditions
Store at -20°C PROTECT FROM MOISTURE.