| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 4-8 weeks |
| Species | Human, Ebolavirus |
| Compatible Sample Types | Plasma, Serum |
| Gene Symbols | IgM |
| Protein Name / Synonyms | IgM; Zaire Ebolavirus Membrane Associated Protein 24 (VP24) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Ebola |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
This in-vitro ELISA kit employs an indirect ELISA method. Standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein-coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-pathogen antigen antibody. A biotinylated anti-positive control antibody is added to the positive control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
| Component | Size / Description |
|---|---|
| Pathogen antigen coated 96-well Microplate | 96 wells (12 strips x 8 wells) coated with pathogen antigen |
| Wash Buffer Concentrate (20X) | 25 ml of 20X concentrated solution |
| Positive Control | 1 vial of Positive Control sample containing anti-pathogen antigen antibody |
| Biotinylated Anti-Human Ig(A/G/M) | 1 vial of solution |
| Biotinylated Anti-Positive Control | 1 vial of solution |
| HRP-Streptavidin concentrate | 1 vial of solution |
| TMB One-Step Substrate Reagent | 12 ml of 3,3,5,5'-tetramethylbenzidine (TMB buffer solution) |
| Stop Solution | 8 ml of 0.2 M sulfuric acid |
| Assay Diluent B | 15 ml of 5X concentrated buffer |
| 5X Sample Diluent | 25 ml of 5X diluent buffer, 0.5% proclin 300 as preservative |
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
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Zaire Ebolavirus VP24 Protein Human IgM ELISA Kit
Zaire Ebolavirus VP24 Protein Human IgM ELISA Kit