AGO2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UKV8 |
| Gene Id | 27161 |
| Gene Symbols | AGO2 |
| Protein Name / Synonyms | AGO2, CASC7, EIF2C2, LINC00980, PPD, Q10, protein argonaute-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
93kDa/97kDa
Observed MW
100kDa
Immunogen
Recombinant protein of human AGO2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AGO2 antibody (144-06802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.