AIFM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95831 |
| Gene Id | 9131 |
| Gene Symbols | AIFM1 |
| Protein Name / Synonyms | AIFM1, AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, apoptosis-inducing factor 1, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
26kDa/28kDa/35kDa/66kDa
Observed MW
67kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AIFM1 antibody (144-02568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/928103fb36b3dcf044239d8f9d25b897cc02663a-628x350.jpg)
Western blot analysis of extracts from normal (control) and AIFM1 knockout (KO) 293T cells, using AIFM1 antibody (144-02568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/a9d919b8a65711777f369f70e354d4183befdc8e-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (144-02568) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/6cf56fe92593a383a5b8e43dcd5b1c57c217d6f0-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/0f632b502588cd5eedd8a3512bf1bd51a59b6f95-350x350.jpg)
Immunohistochemistry of paraffin-embedded human endometrium using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/8fc1bb0143db1936dd2736213e14c8b01a7df366-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse esophagus using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://cdn.sanity.io/images/g51potcz/production/92e309d52f820ca8c7103985338b6096df2dc09b-367x550.jpg)
Immunohistochemistry of paraffin-embedded rat lung using AIFM1 antibody (144-02568) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://www.raybiotech.com/files/images/Antibodies/144/144-02568/7.jpg)
Immunohistochemistry of paraffin-embedded rat liver using AIFM1 antibody (144-02568) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)](https://www.raybiotech.com/files/images/Antibodies/144/144-02568/8.jpg)
Expiration:
12 months from the date of shipment when stored properly.