ANXA1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04083 |
| Gene Id | 301 |
| Gene Symbols | ANXA1 |
| Protein Name / Synonyms | ANXA1, ANX1, LPC1, annexin A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
38kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ANXA1 antibody (144-01118).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)](https://cdn.sanity.io/images/g51potcz/production/84b64cc472cf883b452f38774e10a65b4dac3c80-224x350.jpg)
Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (144-01118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)](https://cdn.sanity.io/images/g51potcz/production/642057679059c68d1dcd3eb7f9ac90a5e33c6336-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (144-01118) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)](https://cdn.sanity.io/images/g51potcz/production/6a21d715c4a84cff3789e9e98ae67e08189969fe-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (144-01118) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)](https://cdn.sanity.io/images/g51potcz/production/129b1fb58f8be35734985c9ff4298449c1e493fb-368x550.jpg)
Immunofluorescence analysis of HeLa cells using ANXA1 antibody (144-01118). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)](https://www.raybiotech.com/files/images/Antibodies/144/144-01118/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.
