CCNG1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P51959 |
| Gene Id | 900 |
| Gene Symbols | CCNG1 |
| Protein Name / Synonyms | CCNG1, CCNG, cyclin-G1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100
IHC 1:50 - 1:100
IF 1:20 - 1:100
Format
Calculated MW
18kDa/34kDa
Observed MW
30kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCNG1 (NP_004051.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCNG1 antibody (144-05292) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunofluorescence analysis of 293T cells using CCNG1 antibody (144-05292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.