CCT8 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P50990 |
| Gene Id | 10694 |
| Gene Symbols | CCT8 |
| Protein Name / Synonyms | CCT8, C21orf112, Cctq, D21S246, PRED71, T-complex protein 1 subunit theta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.
Dilutions
WB 1:1000 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
51kDa/57kDa/59kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 248-497 of human CCT8 (NP_001269837.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CCT8 antibody (144-04449) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot analysis of extracts of various cells, using CCT8 antibody (144-04449) at 1:7000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.