Human CD32 Antibody [clone: IV.3], monoclonal mouse IgG2b. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P12318 |
| Gene Id | 2212 |
| Gene Symbols | FCGR2A, CD32, FCG2, FCGR2A1, IGFR2 |
| Protein Name / Synonyms | Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a) (CDw32) (Fc-gamma RII-a) (Fc-gamma-RIIa) (FcRII-a) (CD antigen CD32) |
| Clonality | Monoclonal |
| Clone | IV.3 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease, Cancer |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with either RayBright® Red 647 Anti-Human CD32 (#136-24068, solid line), or mouse IgG isotype control (dashed line). Monocytes were gated.
Description
CD32 is a polymorphic transmembrane glycoprotein in the immunoglobulin superfamily and is expressed on monocytes, granulocytes, platelets and B cells. CD32 has an affinity to aggregated IgG and immune complexes and has a role in regulating inflammation and immunity.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
